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The SAMs that were formed by a mixture of lipids (DPPTE+EggPC) presented better kinetic parameters and were the chosen to produce tBLMs. Tethered BLMs were made by using the SAM described above as the lower leaflet; the second leaflet was deposited by vesicle fusion, the vesicles were made of EggPC. TBLMs are commonly used as model membranes, however in biophysical studies free standing membranes or ‘black’ lipid membranes are more realistic models of cellular processes.
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